The region is also home to more than 20 colleges and universities, including the University of Rochester, the Rochester Institute of Technology, and the state Universities of New York at Brockport and Geneseo.

Alfred State College
Alfred University
Bryant & Stratton College
Colgate Rochester Crozer Divinity School
Finger Lakes Community College
Hobart and William Smith Colleges
Keuka College
Genesee Community College
Monroe Community College
Nazareth College
Roberts Wesleyan College
Everest Institute
Rochester Institute of Technology
Saint Bernard's School of Theology and Ministry
St. John Fisher College
SUNY Brockport
SUNY Empire State College
SUNY Geneseo
The University of Rochester
Wells College